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KMID : 0350519920450010223
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Journal of Catholic Medical College
1992 Volume.45 No. 1 p.223 ~ p.228
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The Effects of Endogenous Gonadal Steroids on β-Adrenergic Receptor in Human Myometrial Tissue
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Abstract
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The adrenergic response of the myometrium and concentration of adrenergic were influenced by exogenous gonadal sterods in rabbit uterus. In human studies, it was also observed many years ago that the uterine contractile response to catecholamine
stimulation could be modulated by changes in the gonadal steroid hormones.
In order to test the hypotesis that endocrine changes at different pahses of the reproductive cycle and gestation might alter the concentration and affinity of β-adrenergic receptor, the concentration and affinity of β-adrenergic receptor
were-meaured
in myometriums of mid-follicular phase,mid-luteal phase, postmenopause and term pregnancy.
Using the radioligand iodine 125-labeled iodocyanopindolol with high specific activity and high affinity, β-adrenergic receptor concentration was measured.
@ES The results were as follows:
@EN 1. β-adrenergic receptor concentration and dissociation constant in the mid follicular phase(estradiol: 25-75pg/ml, progesterone: 0.4-1ng/ml) were 63.4*22.2 fmol/mg protein and 32.4*13.6pmol/L respectively.
2. β-adrenergic recptor concentration and dissociation constant in the midluteal phase (estradiol; 100-300 pg/ml, progesterone: 5-20ng/ml) were 40.8*12.7 fmol/mg protein and 30.8*11.7 pmol/L respectively.
3. β-adrenergic receptor concentration and dissociation constant in postmenopausal phase (estradiol; 10-20pg/ml, progesterone; <0.1ng/ml) were 52.8*11.0fmol/mg protein and 28.1*8.1 pmol/L respectively.
4. β-adrenergic receptor concentration and dissociation constant at term pregnancy (estradiol; 10-45ng/ml, progesterone; 75-250ng/ml) were 32.5*10.3 fmol/mg protein and 36.9*15.7 pmol/L respectively.
5. The concentration of β-adrenergic receptor in mid-follicular phae were significatly higher than that in the mid-luteal phase and term pregnancy.
6. The concentration of β-adrenergic receptor at term pregnancy were significantly lowere than that of the non-pregnancy myometrium.
But there was no significant difference in dissociation constant(Kd) among the mid-follicular phase, mid-luteral phase,postmenopause and term pregnancy.
These findings suggest that the β-adrenergic receptors in human myometrium might be modulated by endogenous gonadal steroid hormones.
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